You can use Javascripts to validate this as followes.
Define the Javascript Function "isNumberEvt" on Head Section. and check it on keypress even of text box like "onkeypress="return isNumberEvt(event)"".
Javascript :
function isNumberEvt(evt)
{
var charCode = (evt.which) ? evt.which : event.keyCode
if ((charCode > 31 && charCode <> 57 && charCode <> 90 && charCode <> 122))
return false;
return true;
}aspx:
call function onkeypress="return isNumberEvt(event)" even of Textbox.
asp:textbox id="TextBox1" onkeypress="return isNumberEvt(event)" runat="server" width="238px"